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. 2008 Aug 29;283(35):24202–24211. doi: 10.1074/jbc.M804212200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — A, Western blotting of the cerebella of tg(PrPN-Dpl)/Prnp^0/0, tg(PrPΔpreOR)/Prnp^0/0, and tg(PrPΔOR)/Prnp^0/0 mice. 30 μg of the total proteins were loaded onto each lane. Lane 1, Zrch I Prnp^0/0 mice; lane 2, wild-type mice; lane 3, Zrch I Prnp^0/+ mice; lane 4, tg(PrPΔpreOR)/Prnp^0/0 mice; lane 5, tg(PrPΔOR)/Prnp^0/0 mice; lane 6, tg(MHM2Δ23-88)/Prnp^0/0 mice; lane 7, tg(PrPN-Dpl)/Prnp^0/0 mice. B, in situ hybridization of the cerebella of wild-type, Zrch I Prnp^0/0, tg(PrPN-Dpl)/Prnp^0/0, tg(PrPΔpreOR)/Prnp^0/0, and tg(PrPΔOR)/Prnp^0/0 mice. Purkinje cells in Zrch I Prnp^0/0 mice show background staining with the PrP cRNA probe. In contrast, strongly stained Purkinje cells are observed in wild-type, tg(PrPN-Dpl)/Prnp^0/0, tg(PrPΔpreOR)/Prnp^0/0, and tg(PrPΔOR)/Prnp^0/0 mice. Magnification, ×10; inset magnification, ×50. C, Western blotting of the PNGase F-treated homogenates of the cerebella from wild-type (lane 1, 100 μg of the total proteins), Ngsk Prnp^0/0 (lane 2, 100 μg), Ngsk Prnp^0/+ (lane 3, 100 μg), tg(PrPN-Dpl)/Prnp^0/0 (lane 4, 200 μg), and tg(Dpl32)/Prnp^0/0 mice (lane 5, 100 μg) using anti-Dpl FL176 antibodies.