FIGURE 4.
Trajectories of single Kd-peptide P1 complexes on RMA-S-Kd cells. A, diffusion coefficients (D) of single complexes (red bars, 94 molecules on 17 different cells). B, histogram of the confinement lengths (L) of complexes (red bars). Complexes with length >1 μm are represented by a bar at >1 μm. Black bars represent immobilized ATTO molecules and blue bars molecules following simulated Brownian diffusion. C, using length of 360 nm as threshold showed that 16 ± 8% of the molecules were confined, and 84 ± 8% were mobile. D-F, Kd-peptide P1 complexes on RMA-S-KdGPI cells. D, diffusion coefficients (D) of single complexes (red bars, 100 molecules on 18 different cells). E, confinement lengths (L)(red bars). Complexes with length of >1 μm are represented in a bar at >1 μm. F, using the length of 360 nm as threshold shows that 1 ± 2% of the molecules were confined and 99 ± 2% mobile.