FIGURE 1.
Procedures for identification of the SmcR binding sequences. A, the DNA fragments obtained by partial digestion of genomic V. vulnificus DNA with Sau3AI were ligated with linkers at both ends (shown as black tails). Putative SmcR target DNA was enriched in two consecutive cycles of DNA-SmcR interaction, purification of the complexes on Ni2+-nitrilotriacetic acid-agarose, and PCR amplification. B, each DNA released from the DNA-SmcR complexes was cloned, subsequently radiolabeled, and then used for EMSA. The sequences for binding of SmcR to individual DNA were also identified by DNase I footprinting. B, bound DNA; F, free DNA.