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. 2008 Aug 29;283(35):23610–23618. doi: 10.1074/jbc.M801480200

FIGURE 6.

FIGURE 6.

Verification of SmcR binding to the regulatory region of newly identified genes as SmcR regulon. Ten genes were randomly chosen from the pool of the newly predicted SmcR regulon members, and binding of SmcR to their upstream region was examined by EMSA. Each of the DNA fragments was radiolabeled, mixed with 200 nm SmcR, and then resolved on a 4% polyacrylamide gel. EMSAs were performed in the presence of 0.1 μg of poly(dI-dC) as a nonspecific competitor. For details, see “Experimental Procedures.” –, without SmcR; +, with SmcR. The same locus tag numbers that appear in Table 2 and supplemental Table 1 are at the top of each panel. B, bound DNA; F, free DNA.

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