FIGURE 2.

mAbs 8B2 and 7G8 epitopes. The localization of mAbs 8B2 and 7G8 epitopes is shown on the three-dimensional x-ray structure of HCV RdRp complex with oligonucleotide RNA (Protein Data Bank accession code 1nb7, HCV J4 strain (45)). A and B, ribbon representations of the back view are shown. Solid molecular surface representations of back and top views are shown in C and D. The spatial orientation of HCV RdRp is identical for A-C. The epitopes of mAbs 8B2 (amino acids Ser¹-Ala⁹) and 7G8 (amino acids Thr⁹²-Ala¹⁰⁵) are colored red-orange and magenta, respectively. Catalytic aspartate residues are represented as spheres, and their oxygen atoms are displayed as red. Manganese atoms are shown as spheres and depicted in purple. B, Fingers, Palm, and Thumb are depicted in cyan, gold, and green, respectively. Two loops emanating from Fingers subdomain and making extensive contacts with Thumb are designated Λ1 (amino acids Ile¹¹-Ala⁴⁵) and Λ2 (Met¹³⁹-Ile¹⁶⁰) and depicted in dark cyan. The RNA, colored blue, is represented as sticks embedded in transparent mesh molecular surface. Figures were prepared with UCSF, Chimera package (64, 65).