FIGURE 6.

The binding of HCV subgenomic RNA by NS5B is enhanced in the presence of mAb 8B2. RNA binding assays were performed with a ³²P-labeled poly(rC)/(rG)₁₂ template-primer RNA (A) or HCV Con1/luc replicon RNA (B) in the presence of increasing concentrations of mAbs. The NS5B-RNA complexes were immobilized onto nitrocellulose filters (BA85, Schleicher & Schuell), and bound radioactivity was measured by liquid scintillation counting. The means of replicate (three) independent experiments with standard deviation values are indicated.