TABLE 2.

Summary of replication assays results

Mean values from each set of replicate experiments are shown, normalized to the median activity value of either wild-type HCV NS5B polymerase or adaptive Con1/luc HCV replicon controls. In each case, the standard deviation value was less than 10% of the median activity value.

Mutation in RdRp	RdRp activitya	Mutation effect	Con1/luc activityb	Mutation effect	Phenotypec
	%WT		%WT
T92S	18	Down	4	Down	I
P93A	90	WT-like	67	WT-like	I
P94A	115	WT-like	0	Null	II
H95S	104	WT-like	0	Null	II
S96T	82	WT-like	0	Null	II
A97S	88	WT-like	0	Null	II
R98K	157	Up	0	Null	II
R98I	68	WT-like	0	Null	II
S99T	0	Null	0	Null	I
K100I	20	Down	0	Null	II
F101Y	134	Up	0	Null	II
F101A	89	WT-like	0	Null	II
G102A	32	Down	3	Down	I
Y103F	89	WT-like	98	WT-like	I
G104A	146	Up	125	WT-like	NA
A105S	62	WT-like	60	WT-like	I
WT	100	WT	100	WT	I
D318N	0	Null	0	Null	I

^aThe results of standard primer-dependent RdRp assays obtained with purified mutated enzymes are shown.

^bThe results of transient replication assay obtained with mutated Con1/luc replicons (144 h post-transfection).

^cFor phenotype I, the mutation results in the same effect in both assays (up/up, WT-like/WT-like, down/down, or null/null mutations). For phenotype II, the mutation is non-null in primer-dependent RdRp assay and null in transient replication assay; NA indicates not applicable.