FIGURE 2.
D-loop formation activities of Rad521-212 mutants. A, a schematic diagram of the D-loop formation reaction promoted by Rad521-212. B, increasing concentrations (0, 0.03, 0.06, 0.12, 0.25, 0.5, 1, 2 μm) of wild-type (WT) or mutant Rad521-212 proteins were initially complexed with 1 μm ssDNA (5S-repeat-10-1, 50-mer) (first row). Afterward, negatively supercoiled plasmid DNA (pB5Sarray, 5.3 kilobases) was added, and the reactions were either treated with glutaraldehyde (ternary complexes, second row) or proteinase K (D-loops, third row).