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Oligomeric and monomeric forms of PcrV have similar global structural features. Structural features of PcrVuntreated (bold line), PcrVoligo (gray line), and PcrVmono (thin line) were examined by CD experiments. CD spectra of the different forms of PcrV were recorded in near-UV (A) and far-UV (B) to assess tertiary and secondary structures, respectively. Protein concentrations were 10 and 1 μm for near- and far-UV, respectively.
