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. 2008 Aug 29;283(35):23731–23738. doi: 10.1074/jbc.M801918200

FIGURE 4.

FIGURE 4.

Direct interaction between UCH-L1 and the cytosolic region of LAMP-2A. A, an amino acid sequence of biotin-conjugated peptides is shown. B and C, 10μg of recombinant UCH-L1 and 2 nmol of peptides (control or LAMP-2A peptide) were mixed, and a pulldown assay was performed using streptavidin beads. Precipitants were analyzed by immunoblotting (B). The levels of UCH-L1 relative to input were quantified by densitometry. Mean values are shown with S.E. (n = 3). *, p < 0.05. D, 10 μg of UCH-L1 (wild-type or I93M) and 2 nmol of peptides (control or LAMP-2A peptide) were mixed, and a pulldown assay was performed.