TABLE 1.
Gefitinib and NVP-ADW742 combinations synergistically decrease cell numbers in multiple cell lines
Cells were grown for 3 days in medium with 10% fetal bovine serum in the presence of a dose response of these agents alone or in combination. Live cell numbers at the end of treatment were quantified with MTS reagents.
| Cell line | Cell type | Combination index at EC50a |
|---|---|---|
| A431 | Epidermoid carcinoma | 0.33 |
| BxPC3 | Pancreatic adenocarcinoma | 0.35 |
| MIA PaCa-2 | Pancreatic carcinoma | 0.34 |
| H1299 | Non-small cell lung carcinoma | 0.86 |
| A549 | Non-small cell lung carcinoma | 0.35 |
| MDA-435LM | Ductal breast carcinoma | 0.24 |
| Calu6 | Non-small cell lung carcinoma | 0.71 |
a
Combination indices for decreases in cell numbers caused by combinations of gefitinib and NVP-ADW742 were calculated using CalcuSyn. A combination index of <1 indicates synergism, combination index = 1 indicates additivity, and combination index of >1 indicates antagonism. Data are the average of two independent experiments with duplicate samples.