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Effect of mutations on the secondary structure of mPDF. Far-UV CD spectra of wild-type (WT) and mutant proteins (R77K, R78K, R79K, and R77K/R78K/R79K) in 20 mm phosphate buffer, pH 7.4, were obtained between wavelengths 250 and 187 nm. The Ni2+-nitrilotriacetic acid-purified protein samples (∼0.2 mg/ml) were dialyzed to remove immidazole and were used for spectroscopic analysis.
