TABLE 2.
Effect of PTO modified antisense oligonucleotide on mycobacterial growth
| Treatmenta | Antisense oligonucleotides against region of def gene (5′-3′) | Growth | Number of experiments |
|---|---|---|---|
| % | |||
| None | 100 | 6 | |
| PS-ODN-(34-39) | 100-117b | 101.33 ± 5.36 | 6 |
| PS-ODN-(74-83) | 220-249 | 27.19 ± 1.68 | 6 |
| PS-ODN-(104-111) | 310-333 | 32.70 ± 2.0 | 3 |
| PS-ODN-(147-154) | 439-462 | 31.20 ± 1.87 | 3 |
| PS-ODN-(188-197) | 565-594 | 73.59 ± 7.54 | 6 |
| PS-ODN-(S74-83) | 220-249c | 22.53 ± 1.37 | 3 |
| PS-ODN-(S188-197) | 565-594c | 64.43 ± 2.23 | 3 |
a
Numbers in parentheses indicate amino acids against antisense oligonucleotides designed.
b
Antisense oligonucleotides designed to incorporate mismatch between M. tuberculosis (Rv0429c) and M. smegmatis (0832) def gene sequences.
c
Antisense oligonucleotides designed based on the M. smegmatis def gene.