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. 2008 Aug 29;283(35):24194–24201. doi: 10.1074/jbc.M802869200

FIGURE 3.

FIGURE 3.

Acute treatment with resveratrol has dramatic effects on the p70S6K and eEF2 signaling pathways, while affecting both AMPK and Akt signaling pathways. Representative immunoblot and densitometry of cellular extracts from neonatal rat cardiac myocytes treated with ethanol (Control) or 100 μm resveratrol (Resv) for 1 h. Values are expressed as means ± S.E. and analyzed with Student's one sample t test as control was set to 1. A, cell lysates blotted with anti-phospho-p70S6K (T389) and anti-actin antibodies, *, p < 0.0001 versus Control (n = 4); (B) anti-phospho-p70S6K (T421/S424) and anti-actin antibodies, *, p < 0.001 versus Control (n = 4); (C) anti-phospho-eEF2 (T56) and anti-eEF2 antibodies, *, p < 0.05 versus Control (n = 9); (D) anti-phospho-α-AMPK (T172) and anti-α-AMPK antibodies, *, p < 0.05 versus Control (n = 6); (E) antiphospho-ACC (S79) antibody and peroxidase-labeled streptavidin, with both ACC bands being quantified together, *, p < 0.05 versus Control (n = 7); (F) anti-phospho-Akt (S473) and anti-Akt antibodies, *, p < 0.001 versus Control (n = 4); and (G) anti-phospho-GSK-3β (S9) and anti-GSK-3β antibodies, *, p < 0.01 versus Control (n = 4).