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Stimulatory effect of the PCNA trimer on the uracil excision ability of PfuUDG. A, PfuUDG (20 fmol) was incubated with 100 fmol of uracil-containing DNA substrate (G:U*) and 0-450 nm of wild-type (WT) or a monomeric mutant (D143A/D147A) PfuPCNA, in a reaction containing 0.1 m KCl, as described under “Experimental Procedures.” B, the uracil excision efficiencies are plotted as a function of the PCNA concentration as a monomer.
