FIGURE 2.

Enforced expression of KIF1Bβ induces growth suppression in neuroblastoma-derived cell lines. A, MTT assay. Neuroblastoma-derived SH-SY5Y and NB1 cells were infected with the indicated recombinant adenoviruses, including empty adenovirus (pAdNull) at a 200 multiplicity of infection (MOI)(filled boxes) or left untreated (open boxes). Twenty four hours after infection, infected SH-SY5Y and NB1 cells were seeded at a density of 1 × 10³ cells/96-well plates and allowed to attach. Ninety six hours after infection, 10 μl of MTT solution was added to each well and incubated for 3 h at 37°C (left panel). Right panels show the expression of the indicated splicing variants of KIF1Bβ as examined by immunoblotting (IB) with anti-FLAG antibody. B, colony formation assay. SH-SY5Y and NB1 cells were transfected with an empty plasmid or with the indicated expression plasmids. Forty eight hours after transfection, cells were transferred into fresh medium containing 500 μg/ml of G418 and incubated for 2 weeks. After selection with G418, G418-resistant viable colonies were stained with Giemsa solution, and number of colonies was scored.