FIGURE 6.

Identification of the promoter region of human KIF1Bβ gene and its methylation status. A, nucleotide sequence of 5′-upstream region and a part of exon 1 of human KIF1Bβ gene. +1 indicates the first nucleotide of exon 1. CpG island is indicated by underline. B, luciferase reporter assay. Neuroblastoma-derived SK-N-BE cells were transiently co-transfected with the constant amount of pRL-TK encoding Renilla luciferase cDNA and the indicated luciferase reporter constructs. Forty eight hours after transfection, cells were lysed, and their luciferase activities were measured. C, methylation-specific PCR. Methylation status of the promoter region of KIF1Bβ gene in primary neuroblastomas was examined by methylation-specific PCR. M, methylated; UM, unmethylated; C, control.