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Xanthan production in XcK-complemented strains. A, xanthan production in an XcK mutant expressing mutated GumK compared with wild-type strain FC2. The polymer formed was measured by the cetylpyridinium chloride method. XcK and XcK/pBBRprom are negative controls. XcK/pBBRSK is the mutant strain complemented with wild-type GumK protein (positive control). Bars, S.D. from at least three independent experiments. B, normalization of protein quantities by Western blot.
