TABLE 1.
Analysis of individual cDNA clones obtained from in vitro RNA editing reactions with an RNA template containing a four times repeated cis-element
The table lists the editing status of cDNA clones from two independent assays with two different mitochondrial lysates. A C represents an unedited position, and a T marks a successfully edited position. In Repeat 4, two nucleotides of the cluster of editing sites can be edited, which is indicated by the two nucleotides separated by a slash. In Assay 1, 50% in vitro editing was calculated from the TDG reaction. Confirming this, approximately half of the randomly selected cDNA clones (9 of 19) show no editing. In Assay 2, ∼90% editing was indicated by the TDG analysis. Of the 31 clones investigated for these cloned cDNAs, only two clones have no editing site altered.
| Repeat 1 | Repeat 2 | Repeat 3 | Repeat 4 | No. of clones | Editing events | |
|---|---|---|---|---|---|---|
| Assay 1 | T | T | T | T/T | 1 | 5 |
| T | T | T | T/C | 2 | 4 | |
| T | T | T | C/C | 4 | 3 | |
| C | T | T | T/C | 1 | 3 | |
| T | C | C | C/C | 2 | 1 | |
| C | C | C | C/C | 9 | 0 | |
| Assay 2 | T | T | T | T/T | 1 | 5 |
| T | T | T | T/C | 1 | 4 | |
| T | T | T | C/T | 1 | 4 | |
| T | T | T | C/C | 16 | 3 | |
| T | T | C | C/C | 4 | 2 | |
| C | T | T | C/C | 2 | 2 | |
| T | C | T | C/C | 2 | 2 | |
| C | C | T | C/C | 2 | 1 | |
| C | C | C | C/C | 2 | 0 |