FIGURE 7.

Repair of 1-meA and 3-meC by hABH1(Δ3). A, DNA oligonucleotides containing 1-me(dA) or 3-me(dC) were incubated with hABH3 or hABH1(Δ3) (20 pmol) for 15 min, followed by decomposition to nucleosides and LC/MS/MS quantization. The values are means of triplicates. B, methyl-poly(rA) or methylpoly(rC) were incubated with AlkB or hABH1(Δ3) and treated as in A. The values are means of duplicates. C, repair of carboxyfluorescein end-labeled single-stranded or double-stranded 49-mer DNA oligonucleotides containing 3-me(dC) or a 1-me(dA). The substrates were incubated with hABH1(Δ3) (0-20 pmol) or AlkB (10 pmol) and subsequently cleaved with DpnII restriction enzyme, run on PAGE, and analyzed for fluorescence.