FIGURE 1.

Four members of the M. truncatula PHT1 gene family. A, Southern blot of M. truncatula genomic DNA digested with EcoRI and hybridized at high stringency with an MtPT3 gene-specific probe (lane 1), an MtPT1 full-length cDNA probe (lane 2), and an MtPT5 gene-specific probe (lane 3). Size markers (kb) are shown to the right of the blot. B, diagram of the structure of the MtPT genes indicating the 5′-UTR (open bars), coding sequence (dark gray bars), and 3′-UTR (light gray bars). Introns in the 5′-UTR are indicated by triangles and the size of each intron is indicated above the triangle. Total transcript sizes are indicated in brackets.