Four members of the M. truncatula PHT1 gene family.
A, Southern blot of M. truncatula genomic DNA digested with
EcoRI and hybridized at high stringency with an MtPT3 gene-specific
probe (lane 1), an MtPT1 full-length cDNA probe (lane
2), and an MtPT5 gene-specific probe (lane 3). Size
markers (kb) are shown to the right of the blot. B, diagram
of the structure of the MtPT genes indicating the 5′-UTR
(open bars), coding sequence (dark gray bars), and
3′-UTR (light gray bars). Introns in the 5′-UTR are
indicated by triangles and the size of each intron is indicated
above the triangle. Total transcript sizes are indicated in
brackets.