Subcellular locations of MtPT3 and MtPT5. Onion epidermal cells and
M. sativa leaf epidermal cells expressing GFP and phosphate
transporter:GFP fusion proteins. A and B, brightfield and
corresponding fluorescence image of a partially plasmolyzed onion epidermal
cell expressing free GFP. Arrowhead indicates the cytoplasm and free
GFP in the cytoplasm; C and D, brightfield and corresponding
fluorescence image of a partially plasmolyzed onion epidermal cell expressing
MtPT3:GFP. Arrow indicates GFP fluorescence in the plasma membrane
(pm) and Hechtian strands (h). E, brightfield image
of M. sativa leaf epidermis showing epidermal cells (e).
F–H, fluorescence images of single M. sativa epidermal
cells expressing free GFP and fusion proteins. F, M. sativa epidermal
cell expressing free GFP; G, M. sativa epidermal cell expressing
MtPT3:GFP; H, M. sativa epidermal cell expressing MtPT5:GFP. Nucleus
(n), cell wall (cw), and plasma membrane (pm) are
indicated. Bar: D = 50 μm and is representative of
A–D. Bars in F–H = 10 μm.