TABLE 2.

Binding parameters of [I¹²⁵]cyanopindolol derived from saturation experiments carried out in membrane of HEK293 cells stably expressing β₂AR and treated with different cholesterol-depleting agents Saturation experiments were analyzed by nonlinear regression using the GraphPad-Prism program. B_max and K_d parameters were derived from the curve fitting. Values represent the means ± S.E. of four independent experiments performed in triplicate.

Treatment	n	Bmax	Kd	R2
		pmol/mg	pm
Vehicle	4	6.58 ± 0.46	32.59 ± 6.59	0.89
CD	4	6.84 ± 1.55	26.90 ± 5.95	0.93
Cholesterol oxidase (1 unit/ml)	4	6.54 ± 0.62	43.48 ± 10.47	0.93