Table 1.
Proteins identified in GLT-1 immunoisolates
| Accession number | Molecular weight (Da) | 1 | 2 | 3 | |
|---|---|---|---|---|---|
| Plasma membrane transporters, channels, and receptors | |||||
| Excitatory amino acid transporter 2 | P31596 | 62,090 | 13 | 16 | 16 |
| Sodium/potassium-transporting ATPase subunit α-1 | P06685 | 113,040 | 3 | 4 | |
| Sodium/potassium-transporting ATPase subunit α-3 | P06687 | 111,676 | 9 | 9 | 10 |
| Sodium/potassium-transporting ATPase subunit β-1 | P07340 | 35,186 | 3 | 3 | |
| Translation, chaperones, trafficking, and adaptors | |||||
| 40S ribosomal protein S14 | P13471 | 16,255 | 4 | 2 | |
| 40S ribosomal protein S16 | P14131 | 16,428 | 3 | 3 | |
| 40S ribosomal protein S3a | P49242 | 29,927 | 2 | 3 | |
| 60S ribosomal protein L11 | P62914 | 20,235 | 3 | 2 | |
| 60S ribosomal protein L22 | P67984 | 14,741 | 2 | 2 | |
| 60S ribosomal protein L23 | P62830 | 14,848 | 2 | 3 | |
| Adaptor related protein 2 complex subunit α-1 (AP-2) | P17426 | 107,649 | 8 | 5 | 3 |
| Adaptor related protein 2 complex subunit β-1 (AP-2) | P62944 | 104,537 | 8 | 8 | 7 |
| ADP-ribosylation factor 3 | P61205 | 20,680 | 5 | 5 | |
| ADP-ribosylation factor 6 | P62331 | 20,065 | 2 | 2 | |
| Cullin-associated NEDD8-dissociated protein 1 | P97536 | 136,349 | 6 | 6 | 10 |
| Dynamin-1 | P21575 | 95,912 | 14 | 14 | |
| EH domain-containing protein 3 | Q9QXY6 | 60,854 | 4 | 4 | |
| Heat shock 70 kDa protein 12A | Q8K0U4 | 74,855 | 5 | 4 | |
| Heat shock cognate 71 kDa protein | P63017 | 70,855 | 14 | 12 | |
| Alpha internexin | P23565 | 56,099 | 3 | 8 | |
| Neurofilament light polypeptide | P08551 | 61,492 | 5 | 3 | |
| p130Cas-associated protein (SNAP-25 interacting protein) | Q9QXY2 | 129,729 | 3 | 2 | |
| Reticulon-1 | Q8K0T0 | 83,556 | 3 | 2 | |
| Synapsin-1 | P09951 | 73,970 | 11 | 7 | 12 |
| Synapsin-2 | Q63537 | 63,355 | 4 | 3 | |
| Syntaxin-binding protein 1 | O08599 | 67,554 | 8 | 13 | |
| T-complex protein 1 subunit α (chaperonin Cpn60) | P28480 | 60,343 | 6 | 3 | |
| T-complex protein 1 subunit delta (chaperonin subunit 4) | P80315 | 58,050 | 7 | 4 | |
| T-complex protein 1 subunit gamma | Q6P502 | 60,630 | 3 | 2 | 7 |
| T-complex protein 1 subunit theta (chaperonin subunit 8) | P42932 | 59,538 | 9 | 3 | |
| Vesicle-fusing ATPase (N-ethylmaleimde-sensitive fusion protein) | Q9QUL6 | 82,638 | 19 | 16 | |
| Signaling molecules | |||||
| 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase) | P13233 | 47,252 | 16 | 5 | 11 |
| Guanine nucleotide-binding protein G(o) subunit α 1 | P59215 | 40,052 | 8 | 4 | |
| Mitogen-activated protein kinase kinase 1 (MAP kinase kinase) | P31938 | 43,457 | 2 | 3 | |
| Serine/threonine-protein phosphatase 2B catalytic subunit α isoform | P63328 | 58,628 | 8 | 3 | |
| Serine/threonine-protein kinase DCLK1 | Q9JLM8 | 84,137 | 4 | 8 | |
| Glycolysis | |||||
| Glycogen phosphorylase, brain form | P53534 | 96,715 | 4 | 7 | 7 |
| Hexokinase-1 | P05708 | 102,393 | 6 | 10 | 7 |
| 6-Phosphofructokinase type C | P47860 | 85,704 | 12 | 8 | 3 |
| 6-Phosphofructokinase, muscle type | P47857 | 85,252 | 15 | 6 | 3 |
| Glyceraldehyde-3-phosphate dehydrogenase | P04797 | 35,810 | 9 | 7 | 10 |
| Phosphoglycerate kinase 1 | P09411 | 44,521 | 5 | 10 | |
| Pyruvate kinase isozymes M1/M2 | P11980 | 57,801 | 5 | 4 | 9 |
| Mitochondrial proteins | |||||
| Acetyl-CoA acetyltransferase | P17764 | 44,677 | 3 | 4 | 4 |
| ADP/ATP translocase 1 (solute carrier family 25-member 4) (ANT1) | Q05962 | 32,973 | 8 | 9 | |
| ATP synthase B chain | P19511 | 28,852 | 4 | 4 | |
| ATP synthase gamma chain | P35435 | 30,175 | 4 | 6 | 6 |
| Calcium-binding mitochondrial carrier protein Aralar1 (solute carrier family 25, member 12) | Q8BH59 | 74,554 | 15 | 14 | 7 |
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | P08461 | 67,149 | 5 | 10 | |
| D-β-hydroxybutyrate dehydrogenase | P29147 | 38,184 | 6 | 7 | |
| Dynamin-1-like protein | O35303 | 83,893 | 7 | 8 | |
| Dynamin-like 120 kDa protein | Q2TA68 | 111,292 | 4 | 8 | |
| Isocitrate dehydrogenase (NAD) subunit α | Q99NA5 | 39,596 | 5 | 6 | 8 |
| Isocitrate dehydrogenase (NAD) subunit β | Q68FX0 | 42,336 | 6 | 7 | 8 |
| Mitochondrial glutamate carrier 1 (Solute Carrier Family 25–member 22) | Q9D6M3 | 34,653 | 5 | 4 | |
| Mitochondrial 2-oxoglutarate/malate carrier protein (Solute Carrier Family 25–member 11) | P97700 | 34,227 | 3 | 3 | |
| NADH-ubiquinone oxidoreductase 75 kDa subunit | Q66HF1 | 79,396 | 2 | 3 | |
| Phosphate carrier protein | Q8VEM8 | 39,615 | 6 | 11 | 6 |
| Prohibitin | P67778 | 29,803 | 4 | 5 | |
| Prohibitin-2 | O35129 | 33,296 | 9 | 4 | 6 |
| Pyruvate carboxylase | P52873 | 129,668 | 3 | 3 | |
| Pyruvate dehydrogenase E1 component subunit β | P49432 | 38,964 | 2 | 4 | 7 |
| Sideroflexin-3 | Q9JHY2 | 35,415 | 3 | 5 | |
| Stomatin-like protein 2 | Q4FZT0 | 38,396 | 6 | 3 | |
| Trifunctional enzyme subunit α | Q64428 | 82,497 | 2 | 4 | |
| Ubiquinol-cytochrome c reductase complex core protein 2 | P32551 | 48,380 | 12 | 9 | |
| Voltage-dependent anion-selective channel protein 2 | P81155 | 31,716 | 2 | 2 | |
| Voltage-dependent anion-selective channel protein 3 | Q9R1Z0 | 30,781 | 3 | 2 | |
| Other | |||||
| Adenylate kinase isoenzyme 1 | P39069 | 21,566 | 3 | 2 | 3 |
| Complement C3 precursor | P01026 | 186,444 | 3 | 3 | |
| Myelin proteolipid protein | P60202 | 30,060 | 5 | 3 | |
| Probable ATP-dependent RNA helicase DDX5 | Q61656 | 69,304 | 3 | 3 | |
| Puromycin-sensitive aminopeptidase | Q11011 | 103,336 | 2 | 4 | |
| Vacuolar ATP synthase catalytic subunit A (lysosomal V1 subunit) | P50516 | 68,309 | 4 | 3 | 5 |
GLT-1 was immunoaffinity purified from rat cortical tissue using three different strategies as described in Materials and Methods. Proteins identified in at least two of the purifications and not observed in control immunoprecipitations are listed. Proteins were categorized as described in Materials and Methods. The columns on the right labeled 1, 2, and 3 refer to the particular immunoisolation (see Materials and Methods or the first paragraph of Results). The numbers in these columns refer to the number of unique peptides identified in the experiment.