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. 2008 Dec 12;283(50):34745–34752. doi: 10.1074/jbc.M803197200

FIGURE 1.

FIGURE 1.

BBS tag inserted into the R1a subunit is able to bind BTX without altering receptor pharmacology. A, schematic of R1a subunit showing relative positions for the myc tag (blue) and the BBS (red) and the primary sequence. B, images of fixed GIRK cells transfected with R1aBBSR2 + GFP, α7/5HT3a + GFP and GFP alone after treatment with 3 μg/ml BTX-Rhd. GFP (FITC) and rhodamine channels are shown separately and then merged. C, concentration response curves from GIRK cells transfected with either R1aR2 (▪; n = 8–11) or R1aBBSR2 with (▴; n = 5) or without (•; n = 5) an application of 3 μg/ml BTX-Rhd. D, CGP55845 inhibition curves for R1aR2 (▪) and R1aBBSR2 (•) using an EC50 GABA concentration (n = 5). E, time course of responses to submaximal GABA concentration (10 μm) for R1aR2 (▪; n = 5–8), and R1aBBSR2 with either BTX-Rhd (3 μg/ml) pre-applied (•; n = 6–7) or applied after the first and second GABA applications (○; n = 5–6).