FIGURE 4.

Lipin1 maintains the expression of adipocyte functional genes in 3T3-L1 mature adipocytes. 3T3-L1 cells were differentiated to adipocytes, and cells were transfected with lipin1-siRNA or control siRNA. After that cells were incubated for 2 days and harvested to examine the expression of the gene of interest. The expression levels of lipin1, adipogenic transcription factors, and adipocyte functional genes were analyzed by real-time PCR with primers described in Table 1. Relative mRNA levels of each gene were normalized to the levels of actin and then further normalized to the level of siRNA-transfected cells. The data shown are representative of three independent experiments performed in triplicate.