FIGURE 5.
NBD-PS flip is regulated by the pleiotropic drug resistance genes, PDR1 and PDR3. Early log phase (A600 = 0.2-0.4) PDR1-11 (LKY154), pdr3-11 (LKY152), pdr1Δpdr3Δ (AGY75), and PDR1PDR3 (LKY118 or AGY72) cells were labeled with 10 μm NBD-PS for 1 h at 2 °C and washed three times with 2 volumes of cold SC-azide. Mean fluorescence internalized was measured by flow cytometry. Data presented here are the mean of at least three independent experiments, and the error bars represent the S.D. between experiments.