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. 2008 Dec 12;283(50):34571–34579. doi: 10.1074/jbc.M805807200

FIGURE 2.

FIGURE 2.

UDP-MurNAc-Hexapeptide purification and characterization by mass spectrometry. Shown is purification of UDP-MurNAc-l-Ala-γ-d-Glu-l-Lys(ε-l-Ala)-d-Ala-d-Ala (a) and UDP-MurNAc-l-Ala-γ-d-Glu-l-Lys(ε-l-Ser)-d-Ala-d-Ala (b) by Source 30Q anion exchange. Also shown is negative ion electrospray mass spectrum of UDP-MurNAc-l-Ala-γ-d-Glu-l-Lys(ε-l-Ala)-d-Ala-d-Ala (c)(m/z ratio of 609.25 for the [M – 2H]2– ion and 405.82 for the [M – 3H]3– ion) and UDP-MurNAc-l-Ala-γ-d-Glu-l-Lys(ε-l-Ser)-d-Ala-d-Ala (d)(m/z ratio of 1235.42 for the [M – H]– ion and 617.23 for the [M – 2H]2– ion). Methods are described under “Experimental Procedures.”