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. 2008 Dec 12;283(50):35223–35235. doi: 10.1074/jbc.M802660200

FIGURE 4.

FIGURE 4.

Sec- and SRP-dependent export of PhoA fusions. a, subcellular fractionation of DR473 cells expressing a chimeric fusion between the first 372 amino acids of MBP and mature PhoA. Cells were grown in either the presence of arabinose (Ara+) or glucose (Glu+), as indicated; cytoplasmic (cyt) and periplasmic (per) samples were immunoblotted with monoclonal anti-PhoA followed by polyclonal anti-GroEL. b, subcellular fractionation of DR473 or DR473 cells deficient in tatC expressing a chimeric protein fusion of the first 86 amino acids of DsbA to the N terminus of the mature domain of PhoA. Cells were grown in the presence of arabinose or glucose; cytoplasmic and periplasmic samples were immunoblotted with monoclonal anti-PhoA followed by polyclonal anti-GroEL.