FIGURE 4.
Relationship between FRAP recovery kinetics and molecular weight of the AcGFP1-tagged CI subunits in living cells. The kinetics of the fast (tfast) and slow (tslow) recovery phase of the FRAP curve were quantified by fitting a two-component model. A, plot of average tfast (mean ± S.E.) of fusion proteins versus their MW. The values for GFP and GFP2 are on a line through the origin (p < 0.05). B, SDS-PAGE fluorograms (f) and anti-EGFP antibody immunodetections (a) of cells expressing different AcGFP1-tagged subunits. Filled arrowheads indicate fluorescent products of the expected MW. C, plot of average tslow(mean ± S.E.) of subunits versus their MW. The values for GFP and GFP2 are on a line through the origin (p < 0.05). Blue lines indicate 95% confidence limits of the fit lines.