TABLE 1.

FRAP curve fitting results of HEK293 cells expressing AcGFP1-tagged mitochondrial control proteins and complex I subunits

Cell line	MWa	Nb	R2c	tfastd	tslowe	F0f	Fmg
AcGFP1-tagged control proteins
GFP	27.9	171	0.997	0.216 ± 0.009h	1.12 ± 0.034	39 ± 1.1	1.01
GFP2	55.8	58	0.997	0.442 ± 0.024	2.42 ± 0.087	27 ± 1.2	0.99
ANT	62.8	55	0.969	n.d.	8.04 ± 0.644	29 ± 1.7	0.63
AcGFP1-tagged complex I subunits
NDUFV1	77.5	22	0.882	0.401 ± 0.084	5.04 ± 1.20	36 ± 3.5	0.58
NDUFV2	52.6	39	0.981	0.326 ± 0.031	3.26 ± 0.218	37 ± 1.5	0.63
NDUFS3	55.3	43	0.928	0.364 ± 0.061	5.41 ± 0.896	42 ± 2.3	0.52
NDUFA2	39.7	20	0.920	0.230 ± 0.057	1.58 ± 0.213	39 ± 3.3	0.72
NDUFA12	46.0	32	0.971	0.176 ± 0.028	2.30 ± 0.125	36 ± 1.6	0.66
NDUFB6	44.3	57	0.960	n.d.	8.39 ± 0.90	30 ± 1.9	0.46

^aPredicted MW (in kDa), including size of linker (2 kDa) and AcGFP1 (27.9 kDa)

^bTotal number of mitochondria analyzed on at least 2 different days

^cCoefficient of determination, being the proportion of variability in the average FRAP curve that is accounted for by the fit model (calculated using Eq. 2)

^dTime constant of fast FRAP recovery phase in seconds (larger means slower). n.d., not determined

^eTime constant of slow FRAP recovery phase in seconds (larger means slower)

^fStarting level of fluorescence recovery (% of pre-bleach level)

^gMobile fraction (calculated using Eq. 3)

^hStatistics: errors indicate S.E.