TABLE 2.
Antibodies that were used in this study
Antibody name | Origin | Dilution | Manufacturer |
---|---|---|---|
Anti-phospho-ERK | Mouse | 1:2,000 | Santa Cruz Biotechnology (SC7383) |
Anti-ERK | Rabbit | 1:1,000 | Santa Cruz Biotechnology (SC154) |
Anti-phospho-Thr | Mouse | 1:3,000 | Cell Signaling (9386S) |
Anti-phospho-Tyr | Mouse | 1:100 | Millipore (4G10) |
Anti-HA (for Western blot) | Mouse | 1:1000 | Santa Cruz Biotechnology (12CA5) |
Anti-HA (for immunoprecipitation) | Goat | Bethyl Laboratories (A190207A) | |
Anti-His (for Western blot and immunoprecipitation) | Mouse | Sigma (H1029) |