FIGURE 2.

Pseudopodial RNA translocation is microtubule-independent and ROCK and MLC2-dependent in MSV-MDCK-INV cell pseudopodia. A, MSV-MDCK-INV cells were either untreated (control, CTL); treated with the ROCK inhibitor Y27632 (20 μm), the myosin II inhibitor blebbistatin (7 μm), or the microtubule depolymerizing agent nocodazole (2 μm); or treated with both Y27632 and nocodazole or blebbistatin and nocodazole for 90 min. RNA was labeled with Syto14 and β-actin with specific antibodies, as indicated. The arrowheads point to actin-rich pseudopodia. B, pseudopodia were scored for the presence of RNA staining with Syto14 for all conditions (n > 30; ± S.E.; ^*, p < 0.001). Scale bar, 20 μm.