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. 2008 Dec 12;283(50):34785–34795. doi: 10.1074/jbc.M804014200

FIGURE 3.

FIGURE 3.

ROCK-dependence of RNA but not actin turnover in MSV-MDCK-INV cell pseudopodia. A, MSV-MDCK-INV cells stably expressing pEGFP-actin (β-actin-GFP) and untransfected MSV-MDCK-INV cells labeled with the cell-permeant dye Syto14 (Syto14 RNA) were either left untreated (control) or treated with Y27632 for 60 min (+Y27632). Cells were imaged prior to photobleaching (prebleach), immediately after photobleaching of the pseudopodial region with the 488-nm laser (bleach), and 90 s after photobleaching (postbleach). A representative graph of three experiments shows the fluorescence intensity in the bleached region over time (t = 0 corresponds to the time immediately after photobleaching) for β-actin-GFP (B) or Syto14 labeled RNA (C). Untreated cells (control) are shown in blue, and Y27632-treated cells are in red. The values have been normalized to the prebleach intensity. The mobile fraction, representing the percent of fluorescence recovered in the bleached region, and half-time of recovery of control and Y27632 treated cells are shown in graph form for β-actin-GFP (B) and Syto14 RNA (C) (means ± S.E.; n = 3; *, p < 0.01). Scale bar, 20 μm.