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. 2008 Dec 12;283(50):35140–35153. doi: 10.1074/jbc.M804540200

FIGURE 2.

FIGURE 2.

YY1 occupies, both in vitro and in vivo, the -333/-322-bp site of the rat Mmp-9 promoter in the unstimulated rat hippocampus and is released as a result of neuronal depolarization. A, hippocampal nuclear proteins extracted from the unstimulated (-PTZ) rat hippocampi bind to the -333/-322-bp site of Mmp-9 promoter in vitro. B, in vitro binding of rat hippocampal nuclear proteins to the footprinted -333/-322-bp site of the rat Mmp-9 promoter is dependent on neuronal activity status being strongly reduced by neuronal depolarization. The specifically shifted band is indicated with the arrow indicated as “specific band; probe, free (nonincubated with proteins) probe; -PTZ, control; +PTZ, 2 h after PTZ-evoked neuronal excitation. C, EMSA supershift analysis revealed a strong binding of rat hippocampal YY1 to the -333/-322-bp region of Mmp-9 promoter in the unstimulated hippocampus (-PTZ) and its reduction at 2 h after PTZ (+PTZ). YY1 α, supershift with anti-YY1 antibody; no α, EMSA reaction with no antibody included; isotype α, control reaction conducted with an isotype antibody.