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. 2008 Dec 12;283(50):35140–35153. doi: 10.1074/jbc.M804540200

FIGURE 4.

FIGURE 4.

In the rat hippocampus in vivo, neuronal depolarization triggers YY1 and HDAC3 dissociation from chromatin of the proximal Mmp-9 promoter as well as a hyperacetylation of histones H3 and H4 in this genomic fragment. A, neuronal depolarization triggers YY1 dissociation of the proximal Mmp-9 promoter in vivo. Evaluation was by real time PCR of the proximal and the distal Mmp-9 promoter contents as well as levels of the genomic fragment located downstream to the Mmp-9 gene promoter in DNA samples obtained because of immunoprecipitation of chromatin-bound YY1 from the unstimulated (-PTZ) as well as from the PTZ-stimulated (2 h, +PTZ) rat hippocampi. Control ChIP reactions were performed with an isotype antibody. Error bars represent the mean ± S.D. (n = 10; **, p < 0.01). B, neuronal depolarization induces hyperacetylation of histone H3 in the proximal Mmp-9 promoter of the rat hippocampal chromatin in vivo. Evaluation of Mmp-9 proximal promoter content by real time PCR in DNA samples obtained because of immunoprecipitation of chromatin-bound hyperacetylated histone H3 from the unstimulated (-PTZ) as well as from the PTZ-stimulated (2 h; +PTZ) rat hippocampi. Error bars represent the mean ± S.D. (n = 3; **, p < 0.01, ***, p < 0.001). C, neuronal depolarization induces hyperacetylation of histone H4 in the proximal Mmp-9 promoter of the rat hippocampal chromatin in vivo. Evaluation of Mmp-9 proximal promoter content by real time PCR in DNA samples obtained because of immunoprecipitation of chromatin-bound hyperacetylated histone H4 from the unstimulated (-PTZ) as well as from the PTZ-stimulated (2 h; +PTZ) rat hippocampi. Error bars represent the mean ± S.D. (n = 3; ***, p < 0.001). D, HDAC3 occupies the proximal Mmp-9 promoter in unstimulated hippocampus and is released after PTZ-stimulated neuronal depolarization. Evaluation by real time PCR of the proximal Mmp-9 promoter content in DNA samples obtained because of immunoprecipitation of chromatin bound to HDAC3 from the unstimulated (-PTZ) as well as from the PTZ-stimulated (2 h, +PTZ) rat hippocampi. Control ChIP reactions were performed with isotype antibody. Error bars represent the mean ± S.D. (n = 3; *, p < 0.05, **, p < 0.01).