FIGURE 8.

Effect of MPK38 on H₂O₂-mediated apoptosis. HEK293 cells were transiently transfected with increasing amounts of wild-type MPK38 (0.8 and 1.6 μg) and ASK1 (0.5 and 1 μg) or MPK38 and ASK1 siRNAs (100 and 200 nm) in the presence or absence of H₂O₂. Apoptotic cell death was determined using the GFP expression system (GFP) or TUNEL. Cells exposed to 1 mm H₂O₂ for 9 h was used as a positive control. Data shown are means (±S.E.) of three independent experiments. p < 0.05 relative to control; significance calculated by Student's t test.