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. 2008 Dec 12;283(50):34873–34879. doi: 10.1074/jbc.M803207200

FIGURE 1.

FIGURE 1.

Immunodetection of the NDH complex in the BN gel. A, analysis of the thylakoid protein complexes by BN-PAGE. Freshly isolated thylakoid membranes from WT Arabidopsis plants were solubilized in 1% DM at a chlorophyll concentration of 1 μg μl-1, and the protein sample was separated by 5–12% BN-PAGE. The positions of molecular markers are indicated under the gel. The major protein complexes were assigned on the basis of the results of immunoblotting in this study and published information (28). B, thylakoid membrane complexes separated by BN-PAGE in A were further subjected to 12.5% SDS-PAGE, and the proteins were immunodetected with specific antibodies against NdhH, NdhL, PsaA, and CP47, respectively.