FIGURE 4.

Effects of chaperonin complexes on thermal aggregation of CS in the presence and absence of ATP. CS from the porcine heart was incubated at 50 °C for denaturation in the presence of chaperonin complexes (open circles, without chaperonin; closed circles, CpnWT; open triangles, CpnΔhelical; closed squares, Cpn(WWWH)₄; closed diamonds, Cpn(WH)₈; open diamonds, Cpn(WWHH)₄) without (A) or with ATP (B) under potassium-free conditions. The experimental details are described under the “Experimental Procedures.” a.u., arbitrary units. Wild-type and mutant subunit are shown as open and closed circles in the structural images as Fig. 1A.