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Protease digestion assay for the conformational change of chaperonin complexes. Chaperonin incubated with or without ATP (1 mm) was exposed to thermolysin (1 ng/μl) and then analyzed by SDS-PAGE. The arrow indicates the band for the chaperonin subunit. The experimental details are described under the “Experimental Procedures.” Wild-type and mutant subunit are shown as open and closed circles in the structural images as in Fig. 1A.
