TABLE 2.

Fluorescence polarization parameters of chaperonin complexes composed of wild-type and mutant subunits Wild-type subunit, mutant subunit, and fluorophore are shown as open circles, closed circles and asterisks in the structural image. Fluorescence polarization analysis was performed as described under “Experimental Procedures.” The polarization value, a ratio of light intensities, is expressed in millipolarization units, mP (1 polarization unit = 1000 milliunits). Error values of fluorescence polarization are estimated to be under ±4.19 mP (n = 12).