FIGURE 2.

Direct association of SK1 with PP2Ac. Purified recombinant SK1 (1 μg) was included in GST-pulldown assays with either GST alone or GST-PP2Ac (2 μg). Samples were subjected to SDS-PAGE analysis and bound SK1 was detected via its His tag by immunoblotting with an anti-His antibody (upper panel). Coomassie Blue staining was used to confirm the amounts of GST and GST-PP2Ac in each pulldown (lower panel). Results are representative of three independent experiments. The dividing lines indicate where lanes have been spliced to simplify viewing, but results in each panel are from a single experiment.