FIGURE 7.
PP2A mediates deactivation of endogenous SK1 activity following TNFα stimulation. HEK293 cells were transfected with vectors encoding HA-tagged PP2Ac (○), catalytically-inactive PP2AcR89A (▾), or the corresponding empty vector (EV) (•). The cells were stimulated with TNFα (2 ng/ml) 24 h after transfection, for 0, 10, 20, or 40 min, harvested and analyzed for endogenous SK1 activity. Data are mean (±range) of duplicate determinations from a single experiment, and are representative of results obtained in two independent experiments.