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. Author manuscript; available in PMC: 2012 Jan 17.
Published in final edited form as: Chem Sci. 2011;2(6):1080–1085. doi: 10.1039/C0SC00637H

Figure 6.

Figure 6

Dual modulation STED. (A) The fluorescence emission of Alexa 594 solution with the depletion laser at 550 kW/cm2 modulated at 8 Hz and with the primary laser at 40 W/cm2 modulated at 45 Hz. (B) The sum and difference frequency side bands from dual modulation in the Fourier transform of the emission in (A). (C) The frequency components of Alexa 594 solution at the dual modulation difference (37 Hz, triangle) and sum (53Hz, diamond) frequencies as a function of primary laser power. The primary laser is at 535 nm modulated at 45 Hz and the depletion laser is at 745 nm modulated at 8 Hz.