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The FTIR spectroscopy figure of NHS-FA compared with FA.
Notes: NHS-FA presents two remarkable absorption peaks at 1660 cm−1 due to the N–O bond, and at 1700 cm−1 because of the C=O bond. This phenomenon proves that FA has been successfully transferred into NHS-FA.
Abbreviations: FA, Folate; NHS-FA, N-hydroxysuccinimide ester of folate; NHS, N-hydroxysuccinimide.
