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Factors and their levels in 2-LFD analysis
| Independent variables | Symbol | Coded levels | ||
|---|---|---|---|---|
| −1 | 0 | 1 | ||
| FA-CS concentration (mg/mL) | X1 | 0.5 | 4.25 | 8 |
| Oli-A concentration (mg/mL) | X2 | 0.5 | 5.25 | 10 |
| Mass ratio of FA-CS to TPP | X3 | 0.5 | 5.25 | 10 |
| TPP concentration (mg/mL) | X4 | 0.5 | 1.75 | 3 |
| Crosslinking time (h) | X5 | 0.5 | 2.25 | 4 |
Abbreviations: FA-CS, folate-conjugated chitosan; Oli-A, oligomycin-A; TPP, sodium tripolyphosphate; 2-LFD, two-level factorial design.
