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. 2011 Dec 20;6:3449–3459. doi: 10.2147/IJN.S27166

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© 2011 Wang et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

PMC Copyright notice

Hemolytic assay of human red blood cells after treatment with distilled water, phosphate-buffered solution, and differently functionalized nanohydroxyapatite derivatives for 24 hours. The mean and standard deviation for triplicate samples were reported. The inset shows a photograph of human red blood cells exposed to (A) distilled water, (B) phosphate-buffered solution, (C) nanohydroxyapatite, (D) n-HA-APTS, (E) n-HA-APTS.Ac, and (F) n-HA-APTS.SAH for 2 hours (sample concentration 500 μg/mL), followed by centrifugation.

Note: Statistical significance was calculated using analysis of variance. **P < 0.01.

Abbreviations: n-HA-APTS, nanohydroxyapatite-aminopropyltriethoxysilane; n-HA-APTS.Ac, neutralized nanohydroxyapatite-aminopropyltriethoxysilane; n-HA-APTS. SAH, negatively charged nanohydroxyapatite-aminopropyltriethoxysilane.