Fig. 3. Survivin as a target gene of Ang-2.
(A) Ang-2 up-regulated Survivin expression. HAEC were treated with different concentrations of Ang-2 for 6 hours and VEGF was used as a positive control. Survivin mRNA expression was measured by qRT-PCR. Ang-2 dose-dependently up-regulated Survivin expression. (B) Down-regulation of Ang-2 influenced Survivin expression. HAEC were transfected with 50 nM of control (Scr) or Ang-2 siRNA (siAng2) for 48 hours. Survivin expression was measured. (C) OxLDL down-regulated Survivin expression via Ang-2. HAEC were treated with 100 μg/ml of OxLDL for 24 hours in the presence or absence of 1 μg/ml of Ang-2. Ang-2 significantly attenuated OxLDL-induced down-regulation in Survivin mRNA expression (C= control. * vs. C, p < 0.05, n=3; # OxLDL vs. Ang-2 + OxLDL: p < 0.05, n=3).