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a. Representative total RNA integrity analysis paired frozen and FFPE tissue specimens using Capillary electrophoresis Agilent 2100 bioanalyzer, shows that in the FFPE samples RNA exists primarily as fragments between 200 and 100 bases in length. Left panel: fresh frozen human melanoma tissue. Right panel: matched FFPE tissue. b. Boxplot represents the mean mRNA levels and gene expression between frozen and FFPE samples. In red, reference genes.
